Natto Genome Project
Bacillus subtilis subsp. natto str. BEST195
DNA alignment: BSNT_06031 and BPUM_3407
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Amino acid alignment /
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# Program: needle
# Rundate: Mon 8 Mar 2010 06:26:35
# Commandline: needle
# -asequence dna-align/BSNT_06031.1.24716.seq
# -bsequence dna-align/BPUM_3407.2.24716.seq
# -gapopen 10
# -gapextend 0.5
# -outfile dna-align/BSNT_06031-BPUM_3407.aln
# Align_format: srspair
# Report_file: dna-align/BSNT_06031-BPUM_3407.aln
########################################
#=======================================
#
# Aligned_sequences: 2
# 1: BSNT_06031
# 2: BPUM_3407
# Matrix: EDNAFULL
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 1901
# Identity: 945/1901 (49.7%)
# Similarity: 945/1901 (49.7%)
# Gaps: 664/1901 (34.9%)
# Score: 1868.5
#
#
#=======================================
BSNT_06031 1 TTGAAAACATTAGATGTCCACGCTCTTCACG-------AGGGCATTCAGC 43
.|||||||..|.| ||||| ||..|| |.||.|||.|.|
BPUM_3407 1 ATGAAAACCCTTG------ACGCT-TTTGCGCTAATAAACGGAATTGACC 43
BSNT_06031 44 ATACGATTGAGAAACTGGACA-------AGCAAAAACAACAGCTGGAGAA 86
|.|||.| ||| .||| |||||..|||||||.| .|
BPUM_3407 44 AAACGCT---GAA----TACATTAAAGCAGCAATCACAACAGGT----TA 82
BSNT_06031 87 GC----TGGAAAAA-------AGCGTTGAGCATCTGGCAGGCATGAAGGA 125
|| |.|||||| |.| .||.|||.| || |.|||.||
BPUM_3407 83 GCTCTATAGAAAAACAAATCCAAC--AGATCATTT--CA--CTTGATGG- 125
BSNT_06031 126 TGCGCTGAAAGGAAAAGGC-GGAGATGCCATCCGGACCTTTTACGAGGAG 174
.||.|| |||||.|.|||| |||.|.|||||||||.|||||||....||.
BPUM_3407 126 CGCACT-AAAGGGAGAGGCGGGACAAGCCATCCGGTCCTTTTATACAGAA 174
BSNT_06031 175 TGCCACAAGCCTTTTCTCCTTTTCTTTGGCATGT--TTATCGACGAATAC 222
||.||.|..||.||.||.|..|||||| ||.|| |.||.||.||||||
BPUM_3407 175 TGTCATATTCCCTTCCTACAATTCTTT--CAGGTCGTCATTGAGGAATAC 222
BSNT_06031 223 AAAAAAGTACTGAAACAAACACAGCATGCGATT--TCCTCTGTAGAATCT 270
|..|..|..||||||.|.|||.||||.|||.|| |.|.|| |
BPUM_3407 223 AGCACTGCGCTGAAAAACACAAAGCAGGCGCTTCATGCACT--------T 264
BSNT_06031 271 GACTCCCAT------GGCATGATTGCAGAGGCTTTTCTCTCACACGATGC 314
||.||..|| |||.|.||..||.|.||.|||.|...||||||
BPUM_3407 265 GAATCAAATCAAAACGGCTTTATATCACAAGCATTTATTGAACACGA--- 311
BSNT_06031 315 GAGACATGGGGTCAAGCAT---------------GCACGA-----GAA-- 342
||.| |.|||||.|| |.|||| |||
BPUM_3407 312 ---ACTT--GATCAAGGATTAAAAAAAGCTGAACGAACGATTTCTGAAAT 356
BSNT_06031 343 -GTGACAGAGCAGCTCACTGACGCAGTGAATCGCCAGACATCAGCCATCG 391
||..||||| |||||.|.| |||||||
BPUM_3407 357 CGTATCAGAG---------------GTGAACCAC---------GCCATCG 382
BSNT_06031 392 GC---------CACATCG-TCAGCTTGCCG---GCAGTC-AATGATACCT 427
|| ||.||.| ||| .|||||| |.||.| |||.| ||
BPUM_3407 383 GCAGAATTGGTCATATTGTTCA-TTTGCCGAATGTAGACGAATCA-AC-- 428
BSNT_06031 428 TTTTCCGAATGGAAAC------AGAACA-GGCTGAGCGA---CTGATTAC 467
|||||.|.| |||| |.|.|| |||| ||| ||.|
BPUM_3407 429 TTTTCAGCA---AAACTATCAAAAAGCATGGCT---CGAGACCTCA---- 468
BSNT_06031 468 AGACACC------CTGCATAAGCTCTTTCA---ATTTGACGGCCAGCAAA 508
||| ||| |||| |||| |||||||.|..||||.|
BPUM_3407 469 AGA-ACCATTGGTCTGC---------TTCATGCATTTGACAGAGAGCAGA 508
BSNT_06031 509 CCCAAGCACT-----AGAAGCAAAATCAGATT---TTCAAACGATGAAAA 550
|||||.|| ||||.||||| || || |||||||.|||||..
BPUM_3407 509 --CAAGCGCTTTGCAAGAAACAAAA--AG-TTCCCTTCAAACAATGAAGC 553
BSNT_06031 551 AATACATTGATCAGCTCGAAA---CCATGTACACTGGGCCGAAAATTGAA 597
|||||||..||..|.| || ||||||.|||.||.||.|||||.||.
BPUM_3407 554 AATACATCAATACGTT---AAGTGCCATGTTCACCGGCCCAAAAATCGAC 600
BSNT_06031 598 ATCACAGGCTA-CAAAAGCGGCTCCATCCTGAAATCACAGG--AACAAGA 644
|||||..|||| ||||| .||.||.||..|.||| |..|| ||.||||
BPUM_3407 601 ATCACGAGCTATCAAAA-AGGGTCTATTTTCAAA--AATGGAAAAGAAGA 647
BSNT_06031 645 AAACATCAACCA-AACCTTCGGCGCGATT----------------AACCC 677
|||.|| ||.|| .|||.||.|.| |.|| |||||
BPUM_3407 648 AAAAAT-AAGCAGCACCATCAGTG-GGTTGAATGACAAAATAGACAACCC 695
BSNT_06031 678 GCAAATGAAGCAGCCGGATGATTCACCGATGGAGATGATGCTGAAGAAAA 727
| ||.|| ||.||.|||||| |||.|||||||||||..
BPUM_3407 696 G--AAAGA--CAACCCGATGAT-----------GATCATGCTGAAGAAGC 730
BSNT_06031 728 T-AGAGAAACATAAGCAGTCCAACGTTGATATCGTGATGAAGGAT----G 772
| |||||.|.|.|| |.|.|.||.||.|| ||.||.| |
BPUM_3407 731 TGAGAGAGAAAGAA-CGGACGAATGTGGA---------GACGGTTGTTCG 770
BSNT_06031 773 GCAAACAAC----AG-AAGATCGAGCGTGAGATTCATGCTGATGACAGTA 817
|.|||||.| || ||.||| |.||| |
BPUM_3407 771 GGAAACACCGTCTAGTAAAATC---------AATCA-------------A 798
BSNT_06031 818 ACGTAACTGTATTACAGAAAGAAGCAGCAGCTCACCCGAAAGTCTATGGA 867
|| ||||| |||||
BPUM_3407 799 AC-TAACT---TTACA---------------------------------- 810
BSNT_06031 868 GACATACGGGTGATAAATGATAAACTATATAATAATAAAAGATTA-AAAA 916
.||||||..|||.||||.|| .|||||.||.|| ||||
BPUM_3407 811 ------------TTAAATGGAAAAGTATACAA-CATAAATGAGTATAAAA 847
BSNT_06031 917 AGAT---------AGATACGATAGAAGTGATTGACGAACTAACTC--GCA 955
|||| |.||.|.| ||..|.|||||| || ||.
BPUM_3407 848 AGATCAACTTTGAACATGCCA---AAACGGTTGACG-------TCATGCG 887
BSNT_06031 956 ATACTGCAAGCATCGACTATGTCGGCGGCAAATATTATGTTTATGAA--- 1002
|.||.|.|.| ||.|.||||...|.|||||.| |||
BPUM_3407 888 AGACGGAATG------CTGTATCGGTTACGAATATCA-------GAAAAA 924
BSNT_06031 1003 --------AACG---GCCAGATCGT--AAGAGAATTCTATGCTGGAGGGA 1039
|||| | |||.||| ||..||.|| |||.||.||
BPUM_3407 925 AACAAAGTAACGATTG--AGAGCGTTAAACCGATTT-----CTGAAGTGA 967
BSNT_06031 1040 AAAAGC-GGCTAGAA-----GAAGTGTCTTACATACCAGAGGATAAAGTA 1083
||| | |||.|..| .||.|.|||||.||| |||.||.|.||.|.
BPUM_3407 968 AAA--CAGGCGATCAACCCCAAAATATCTTAAATA-CAGCGGTTGAATTT 1014
BSNT_06031 1084 GGCGGAGCTAAGTCACTGGATTC--ATTTTTGGCTGGAA----------- 1120
..|||| .|||...||..||||| .||.|| .|.||||
BPUM_3407 1015 ACCGGA-ATAAACGACGCGATTCGGGTTATT-ACAGGAAGAGATCCAATA 1062
BSNT_06031 1121 --------------CGC-AGTATGAATTTATTGAGTGGGTAAGCCCTCAG 1155
||| |.||||.||...|| |||| ||||
BPUM_3407 1063 ACAGGTGATAAAGTCGCGAATATGGATCGTTT-----GGTA-----TCAG 1102
BSNT_06031 1156 GG-------TGCTGTT--AA-----AAAATTGGCGGTCCGAGGCGGGAAG 1191
|| || ||| || ||.||| ||...||..|||||
BPUM_3407 1103 GGGTGTACATG--GTTCCAAGCACCAACATT----GTTAAAGTGGGGAA- 1145
BSNT_06031 1192 CGTGTTGTCACAGATGTTGCTAAACATGT---AGTGGAGAAGGATA--TA 1236
|.|||||..||||..|.| ||.||||||.| || ||
BPUM_3407 1146 -----------ATATGTTTTTAAAATTATTAAAGGGGAGAAAG-TAGCTA 1183
BSNT_06031 1237 A-AGGG------AGACGTAAAAAAGAAACCTT-------------CTAAA 1266
| |||| ||| ||..||||| .| |||||
BPUM_3407 1184 AGAGGGTTGAGAAGA-GTGCAAAAG-----GTGCGAGGTATGGGGCTAAA 1227
BSNT_06031 1267 GGCAGTGTTAATAGTAAGTGGTG-----GCATCCTGGATA---TGTAGAT 1308
| |.|.|.||.|.|.|.|.||| ||.|||.|.|.| |||.|
BPUM_3407 1228 G--ATTTTGAAAAATCATTAGTGAAATTGCCTCCAGCAGAGCGTGTCG-- 1273
BSNT_06031 1309 AATTTAAGCTCTTCCCAAACGGTTATAGGAAT-------AAAGAA-TTCC 1350
.||||.| ||| ||| |||||| ||
BPUM_3407 1274 -----CAGCTGT----AAA----------AATGAAAGTAAAAGAAGTT-- 1302
BSNT_06031 1351 CCTAAAGGTTTATCAACTCTTGGTAGCTCAACACGTCAGAATGCACTAGA 1400
.|||||.|| || |.|||.||.| || |||
BPUM_3407 1303 GCTAAAAGT-----AA-------TGGCTTAATA------AA------AGA 1328
BSNT_06031 1401 TGCAGGAAAAGGCTGGGTTGGTCAAGGAGCGGAAAAA--------ATGTA 1442
|.| |||| |.|||.| .||..|| |||||
BPUM_3407 1329 TTC---AAAA-----------TTAAGTA--AGATTAATAGTAGGGATGTA 1362
BSNT_06031 1443 TGATAAAG---------CAGGAAATTTTTTAGGGTACAAAAGTGTAGATA 1483
| |||||| |.|||.||||.| .|.|| |||||||
BPUM_3407 1363 T-ATAAAGATCCTAAAACTGGAGATTTAT----ATTCA-----GTAGATA 1402
BSNT_06031 1484 AA----ATGAGAGCTTTCAGATTGCAATATAAACCAAAGGAAA-----AA 1524
.| |||...|.|||.|.|...||| |||| |||.||.|| ||
BPUM_3407 1403 CACAGCATGGTCGTTTTGAAAAAACAA-ATAA--CAAGGGGAAGCATCAA 1449
BSNT_06031 1525 ATGTGGAGAG--CGAACTTCACTGAAAATGAAATTA---CTGTTGTAGGA 1569
|||||| .||..||.|.|..||| .|||.|| |||.||..|.|
BPUM_3407 1450 ----GGAGAGGTTGATTTTGATTTTAAA-CAAACTAAACCTGCTGATGCA 1494
BSNT_06031 1570 TCAAAAACGGAATTAAGAAATGTACATGTTGATATTTT--AGATTAA--- 1614
|| || .||.|.|||.||| |.|||||
BPUM_3407 1495 TC------------------TG---GTGGTCATAATTTAAAAATTAAGTA 1523
BSNT_06031 1614 - 1614
BPUM_3407 1524 G 1524
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