Natto Genome Project
Bacillus subtilis subsp. natto str. BEST195
Amino acid alignment: BSNT_00626 and BPUM_0321
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# Program: needle
# Rundate: Mon 8 Mar 2010 06:21:24
# Commandline: needle
# -asequence pep-align/BSNT_00626___srfAC.1.24716.seq
# -bsequence pep-align/BPUM_0321.2.24716.seq
# -gapopen 10
# -gapextend 0.5
# -outfile pep-align/BSNT_00626___srfAC-BPUM_0321.aln
# Align_format: srspair
# Report_file: pep-align/BSNT_00626___srfAC-BPUM_0321.aln
########################################
#=======================================
#
# Aligned_sequences: 2
# 1: BSNT_00626___srfAC
# 2: BPUM_0321
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 1282
# Identity: 640/1282 (49.9%)
# Similarity: 885/1282 (69.0%)
# Gaps: 23/1282 ( 1.8%)
# Score: 3364.5
#
#
#=======================================
BSNT_00626___ 1 -----------MYYLSPMQEGMLFHAILNPGQSFYLEQITMKVKGSLNIK 39
||||||||||||||.:.:..:.||:||:.|.|||:|...
BPUM_0321 1 MSSFKRDQVQDMYYLSPMQEGMLFHTLHHQEKGFYVEQMDMNVKGTLRHD 50
BSNT_00626___ 40 CLEESMNVIMDRYDVFRTVFIHEKVKRPVQVVLKKRQFHIEEIDLTHLTG 89
.||:|||||::|||:|||||:|||||||||||||||.|.::.:|:..|:.
BPUM_0321 51 LLEKSMNVIVERYDIFRTVFLHEKVKRPVQVVLKKRPFTLDVVDMQDLSE 100
BSNT_00626___ 90 SEQTAKINEYKEQDKIRGFDLTRDIPMRAAIFKKAEESFEWVWSYHHIIL 139
.||..:|..:|:||::|||||::|..|||.:|:.:..|:.|:||||||:|
BPUM_0321 101 DEQLVRIETFKQQDQLRGFDLSKDALMRATVFQTSPTSYRWIWSYHHILL 150
BSNT_00626___ 140 DGWCFGIVVQDLFKVYNALREQKPYSLPPVKPYKDYIKWLEKQDKQASLR 189
||||||:|||:||.:|:||....||.|.||||||:||:|||||||||||:
BPUM_0321 151 DGWCFGLVVQELFAMYHALLHDIPYKLEPVKPYKEYIQWLEKQDKQASLQ 200
BSNT_00626___ 190 YWREYLEDFEGQTTFAEQRKKQKDGYEPKELLFSLPEAETKAFTELAKSQ 239
||::.|..|:.|:||.||| ||.:.:|..|:.:|:.:.||.|.:.||..|
BPUM_0321 201 YWQQSLAGFDVQSTFKEQR-KQTNEHELGEMEWSMSKEETAALSALAFQQ 249
BSNT_00626___ 240 HTTLSTALQAVWSVLISRYQQSGDLAFGTVVSGRPAEIKGVEHMVGLFIN 289
:.|||:.||:|||:|:||||:|.|:.||||||||||::.||:.|||||||
BPUM_0321 250 NATLSSVLQSVWSILLSRYQRSNDVLFGTVVSGRPADLAGVDKMVGLFIN 299
BSNT_00626___ 290 VVPRRVKLSEGITFNGLLKQLQEQSLQSEPHQYVPLYDIQSQADQPKLID 339
|:|||::|::.:||..||.:.|:|||.:|||||:|:||||::.....|||
BPUM_0321 300 VIPRRIQLTDDMTFRTLLSETQQQSLAAEPHQYIPIYDIQAETGHQPLID 349
BSNT_00626___ 340 HIIVFENYPLQDAKNEESSENGFDMVDVHVFEKSNYDLNLMASPGDEMLI 389
||:||||.|.. .|:|:.:..||.:.:::|:|||||||||:||||:::.:
BPUM_0321 350 HIVVFENVPTA-KKDEQEARLGFTVENMNVYEKSNYDLNLLASPGEQLQL 398
BSNT_00626___ 390 KLAYNENVFDEAFILRLKSQLLTAIQQLIQNPDQPVSTINLVDDREREFL 439
|||:|:..||..|:.|||.||...:.:.|::|||.|.|:.||..:|::.:
BPUM_0321 399 KLAFNKKAFDSQFVQRLKEQLSLLMSEAIKHPDQSVHTLTLVTKQEKQLI 448
BSNT_00626___ 440 LTGLNPPAQAHETKPLTDWFKEAVNVNPDAPALTYSGQTLSYRELDEEAN 489
|...|.....|:...|:.||:..|...|:|.||:....|::|.||:|:||
BPUM_0321 449 LEEWNASELEHDQLYLSKWFEHNVRKQPNAVALSAGEHTMTYAELNEQAN 498
BSNT_00626___ 490 RLARRLQKHGAGKGSVVALYTKRSLELVIGILGVLKAGAAYLPVDPKLPE 539
||||.:||:|....:|:|:...|:.||::.:|.||||||.|:|:||..||
BPUM_0321 499 RLARHMQKNGVTHQTVIAIIADRTSELIVSLLAVLKAGAIYVPIDPDYPE 548
BSNT_00626___ 540 DRISYMLADSAAACLLTHQKMKEQAAELPYTGTTLFIDDQTRFEEQASDP 589
.||.|||.||.|..||||.....|...|.:.||.||.|||......:.:.
BPUM_0321 549 SRIQYMLKDSGATHLLTHSSFISQVKALAFDGTYLFADDQEISLMSSENL 598
BSNT_00626___ 590 ATAIDPNDPAYIMYTSGTTGKPKGNITTHANIQGLVKHVDYMAFSEKDTF 639
......:|.|||||||||||:|||.:|||:||..:||:.:|:..||.||.
BPUM_0321 599 PLEAGLHDTAYIMYTSGTTGQPKGIMTTHSNIARVVKNTNYLTISETDTL 648
BSNT_00626___ 640 LSVSNYAFDAFTFDFYASMLNAARLIIADEHTLLDTERLTDLILQENVNV 689
||:||..||.||||.|.::||.|:|::..:.|:|:...||.||.:||::|
BPUM_0321 649 LSLSNSVFDGFTFDVYGALLNGAKLVLPKKETILNMGELTGLIKRENISV 698
BSNT_00626___ 690 MFATTALFNLLTDAGEDWMKGLRCILFGGERASVPHVRKALRVMGPGKLI 739
||..|||||||.|...|||:.:|.:|||||||||.|||||..|||.|:||
BPUM_0321 699 MFVPTALFNLLVDENTDWMRSVRKVLFGGERASVQHVRKAFAVMGKGRLI 748
BSNT_00626___ 740 NCYGPTEGTVFATAHVVHD-IPDSISSLPIGKPISNASVYILNEQSQLQP 788
|.|||||.|||||.:.|.: :|..:.|:|||||::....|||::..||||
BPUM_0321 749 NVYGPTESTVFATYYPVDEALPMDMRSIPIGKPLNQTGAYILSQHRQLQP 798
BSNT_00626___ 789 FGAIGELCISGMGVSKGYVNRADLTKEKFIKNPFKPGETLYRTGDLARWL 838
.|.:||||:||.|::|||:||.||||:.||.:||...|.||.|||||.:.
BPUM_0321 799 IGMVGELCLSGKGLAKGYLNRPDLTKQAFITHPFAARERLYCTGDLAYFR 848
BSNT_00626___ 839 PDGTIEYAGRIDDQVKIRGHRIELEEIEKQLQEYPGVKDAVVVADRHESG 888
.||.||||||:|||||||||||||.|||..|..:||||.|.::||:|.:.
BPUM_0321 849 ADGLIEYAGRVDDQVKIRGHRIELTEIEAHLLMHPGVKQAALIADQHNTQ 898
BSNT_00626___ 889 DASINAYLVNRTQL--SAEDVKAHLKKQLPAYMVPQTFTFLDELPLTTNG 936
...:.||:....:. ..:.:|:.||::|||||.|.....|:::|||.||
BPUM_0321 899 HTRLLAYMTCEEEWKDKLDVIKSGLKEKLPAYMQPHELIRLEKMPLTPNG 948
BSNT_00626___ 937 KVNKRLLPKPDQDQLAEEWIGPRNDMEETIAQIWSEVLGRKQIGIHDDFF 986
||:||.||||:..|.......|.|::|:.:..:|.|||.|:.|...|.||
BPUM_0321 949 KVDKRQLPKPEVPQGNHHLKLPVNEVEQKLIVMWQEVLERENISTDDHFF 998
BSNT_00626___ 987 ALGGHSLKAMTAASRIKKELGIDLPVKLLFEAPTIAGISAYVKNGGPDGL 1036
.:||||||||:..|::.||..:.:|:.|:||.|||..||.|::....:.
BPUM_0321 999 EIGGHSLKAMSLLSKVSKEFDVQVPIHLMFETPTIEAISRYIQQQDQEA- 1047
BSNT_00626___ 1037 QNVTIMNQDQEQIIFAFPPVLGYGLMYQNLSSRLPSYKLCAFDFIEEEDR 1086
....:.|:.|...:||.||:.|||.:||..:..|...:|.||||||.:.|
BPUM_0321 1048 AGYLVFNESQTPTVFALPPLPGYGFIYQEAAKTLDDVRLIAFDFIEADHR 1097
BSNT_00626___ 1087 LDRYADLIQKLQPEGPLTLFGYSAGCSLAFEAAKKLEEQGRIVQRIIMVD 1136
:.:|...||.|||||||||.|||.||.||||..:.||:.||.|:::||:|
BPUM_0321 1098 ITQYVQHIQHLQPEGPLTLMGYSGGCYLAFELVQSLEQAGRTVEKVIMID 1147
BSNT_00626___ 1137 SYKKQGVSDLDGRTVESDVEALMNVNRDNEALNSEAVKQGLKQKTHAFYS 1186
||.|.|.|||:||:::.|:.|:::.::.:|...:|.::..|.|||.|:|.
BPUM_0321 1148 SYMKIGESDLEGRSIDDDIAAIVHQSKQSELAQAELIQDELAQKTRAYYE 1197
BSNT_00626___ 1187 YYVSLISTGQVKADIDLLTSGAAFDMPEWLASWEEATTGAYRMKRGFGTH 1236
.:|..::.|:::||||.:.|.....:|||:..||.|||||||..:|:|.|
BPUM_0321 1198 TFVKGVNQGEIQADIDFIQSEENIAIPEWMDHWEAATTGAYRQYQGYGQH 1247
BSNT_00626___ 1237 AEMLQG-ETLDRNAEILLEFL---NTQTVTVS 1264
|:|.:. |...|||:::.|.: |.:||.
BPUM_0321 1248 ADMFKNKECAARNAQLINEIVHHKNRETVL-- 1277
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