Natto Genome Project
Bacillus subtilis subsp. natto str. BEST195
Amino acid alignment: BSNT_05334 and BPUM_3141
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# Program: needle
# Rundate: Mon 8 Mar 2010 06:25:55
# Commandline: needle
# -asequence pep-align/BSNT_05334___yvnB.1.24716.seq
# -bsequence pep-align/BPUM_3141.2.24716.seq
# -gapopen 10
# -gapextend 0.5
# -outfile pep-align/BSNT_05334___yvnB-BPUM_3141.aln
# Align_format: srspair
# Report_file: pep-align/BSNT_05334___yvnB-BPUM_3141.aln
########################################
#=======================================
#
# Aligned_sequences: 2
# 1: BSNT_05334___yvnB
# 2: BPUM_3141
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 1314
# Identity: 625/1314 (47.6%)
# Similarity: 873/1314 (66.4%)
# Gaps: 54/1314 ( 4.1%)
# Score: 3251.0
#
#
#=======================================
BSNT_05334___ 1 MRKYTVIAS---ILLSFLSVLSGGHHESKAFPVVQQEL-FQTPHYIPLLE 46
.|..| :||.|..:|...:..:.|.|.:|.:: .....:.|.:.
BPUM_3141 1 ----MVNVSRQIMLLCFFIILFCFNQPASAGPFIQTQVNIPDKPFSPFIF 46
BSNT_05334___ 47 NPPQIDVK---AEMRSNLMIEAQVRDNSYQAFSAFLFYKQSNELGYKMVP 93
:|..:||: .|...:|...|.:.|.. |..||.|:||||:||||:::|
BPUM_3141 47 DPFHVDVRPVLGEKSRDLQFRASITDQE-QILSANLYYKQSDELGYRLIP 95
BSNT_05334___ 94 MDPTPGAVRKFLAQIPKRLIWNSELEYYIVMSNGKQRVESETKKLKLEGY 143
|:..||...:|.|:||...:||..:.||......|..:::..:.:.|.|:
BPUM_3141 96 MERLPGMKPEFTAKIPPDQLWNPTVRYYTEFLTQKSVIKTAEQSVHLNGF 145
BSNT_05334___ 144 QADLAHIPELLITELAVDTKNIGRADGYEFIEIFNTTDRTIDFKDYHIRY 193
:.||:.||:|:|||:.|::||....|.:||||::|||...|..|.|.:||
BPUM_3141 146 EEDLSRIPDLVITEIVVNSKNRKGKDAFEFIEVYNTTHEPISLKHYQLRY 195
BSNT_05334___ 194 RYPKEGPDSDLIW----RPDERILIPSGETFVVWLKPAGHPELTSEDFNR 239
|.|.:|.:.|.:: :| :.:..|:.:|.||:..|...||..|||.
BPUM_3141 196 RNPAQGTEMDDVYSFHTKP---VTLSPGKPYVFWLRKKGQEHLTVADFNA 242
BSNT_05334___ 240 YYQTQLKEGKNLAVIDETEGMANTRPRAVVISTNTGKDISVAHYR----- 284
.|::|||||::::|| |::|::|::.|.|.|:||.|::||.|.:|
BPUM_3141 243 MYKSQLKEGEHVSVI-ESKGLSNSKSRLVSITTNIGEEISSAAFRLKPSW 291
BSNT_05334___ 285 --KHALRRLSSVLYKYPLNGTAELLNISIGEKNPSPGTVLQAQVPEQKRK 332
:| .|:|:|||::||:::..||..|:.|:||.||:||:|.:|::
BPUM_3141 292 FSEH-----KSLLFKYPMDGTSQMKLISKIEEEPTPGKVLKAQIPSEKKE 336
BSNT_05334___ 333 IKLDKEKPVIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENK 382
:..|..||||||:|.||||:|.||||::||::|..:|||....|||:...
BPUM_3141 337 MSQDIHKPVIEDMTVRKPVKPTESIEIKADVQDDQVVKTVKLRYRTNRKD 386
BSNT_05334___ 383 PFKRILAEKDRNDNLYHYIVYSPELIGKDQLEYYVAAGDGINEARTPVKM 432
.||.||.:||.||.|:|:|.||||||||:||||.:.|.||.|.|.|..||
BPUM_3141 387 DFKEILLQKDHNDRLFHHIFYSPELIGKEQLEYQLEASDGENTAMTMKKM 436
BSNT_05334___ 433 IDIKQTSKAHGLRMNIENRDTLSGTQFLKATTEGRADSIKLWIDGK-KQV 481
||::|.||:||||.|:|..||:||...||||:: :..||:|.|||| ||.
BPUM_3141 437 IDVEQVSKSHGLRFNVEEGDTVSGLFPLKATSD-QKKSIELLIDGKRKQQ 485
BSNT_05334___ 482 TEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVFDDTTNKYRTYSVPL 531
...|:|.:.|.|||.:.|||||||.|.:..::|.:|||:|..||||::|:
BPUM_3141 486 VFSALEADPYIAFDVKGTNLYFKNGVFIGKRLLSIFDDSTKVYRTYTMPI 535
BSNT_05334___ 532 PETLLRKGKQLQRITIRSGSKVSPFD--TAENRDDFLVKNARLVLSDGTV 579
||..::|. .||.|:..:|:|.||.| ..||.|||.:||.|::||:||.
BPUM_3141 536 PEAEMKKA-GLQTISFHAGTKSSPVDRLPGENHDDFELKNVRMILSNGTQ 584
BSNT_05334___ 580 IRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEG 629
|......|:||..:|||..:.|...|||.:|...:.|..::|||.:|.||
BPUM_3141 585 IPPINAMPQKEWRMGDNSNAKKVRTFQFEIPKKAYVSTYMKWDTRRLKEG 634
BSNT_05334___ 630 AHHIQASDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKW 679
.|.|:|....|:||:.|.|||:||.|:.|:.....|||...|:|.:.|:|
BPUM_3141 635 PHLIEAKTSDEHVSVHVMVDNTGPKIKTNVQTKDPYKGAFTLKAKVTDRW 684
BSNT_05334___ 680 SKIEEAEASLDGESITLPYHTSSSDLLPGKHSLKVTATDLAGNKTVIERI 729
|.|||.:|.:||:.|:|||.||||:|.||:|:|.:.|.|.|||.:.:::.
BPUM_3141 685 SDIEEMKAYVDGKPISLPYQTSSSELNPGEHTLTIKAKDAAGNMSTLKKE 734
BSNT_05334___ 730 FKTEREHPDQPEVIDSEADTHKAKLSVRVKDPTNDAMDVGFYRGFQYTAR 779
|||..|||:.||.:..:.....:|.:|..||||||.:|..|||.:|||:.
BPUM_3141 735 FKTVHEHPEPPERVADKTKMKSSKQTVLTKDPTNDKLDYSFYRAYQYTSL 784
BSNT_05334___ 780 N-HVKIFKHASLTEPPKSFVPERETPFTNKELERVSAADGKTVSTENKEL 828
: .:||.|:|:.|||||:|..:.|..|:.:|..|::.||||:|.|::...
BPUM_3141 785 HPSMKILKYAAETEPPKNFNEKGEMIFSTQERNRIAFADGKSVETKDLHR 834
BSNT_05334___ 829 FPYHRFEVTVDPSIDENDLAEAVWKGSSLPGRKVTMYAWNYRTNEWQPVD 878
||||||::.||.::.|.|:.||:||||||.||||||||||::..:|..:.
BPUM_3141 835 FPYHRFQIKVDQAVREKDVVEAMWKGSSLSGRKVTMYAWNHQQEKWDKIT 884
BSNT_05334___ 879 SFVAKDDKPFTLKASVIAADFVRESKMNVIVQDEIPPAKDMYTFVWMSDT 928
...|||.|.|.||..:....:::....:::|||||..|:..|..||||||
BPUM_3141 885 HHFAKDQKEFYLKGLLSVRQYMKNHTFDLLVQDEISKAQKPYKMVWMSDT 934
BSNT_05334___ 929 QYYAESYPHIFDKQTEWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNAD 978
||||:||||||.:..|||:|.:::|||:||||||||||||.| :||..||
BPUM_3141 935 QYYAKSYPHIFKQMVEWIRDQREKLNIQYVFHTGDIVDDSKD-KQWHRAD 983
BSNT_05334___ 979 RSMSVLDKSGIPYGVLAGNHDVGHKDGSYRVFGKYFGSDRFDKKLHYGGS 1028
..|.|||:..||||||||||||.||||.|..:|||||..||..|.:||.|
BPUM_3141 984 TFMRVLDEHQIPYGVLAGNHDVRHKDGFYAEYGKYFGEKRFANKPYYGES 1033
BSNT_05334___ 1029 YKNNRGHYDLISSNGNDYIMLYMGWGITDEDIAWMNQVLKKHPDRMAILA 1078
|:||||||||||:.||||:|:||||||..::|.|:|:|:.:||||:.||.
BPUM_3141 1034 YQNNRGHYDLISAGGNDYMMVYMGWGIKQDEIDWLNRVISEHPDRIVILN 1083
BSNT_05334___ 1079 FHEYLLVSGNRSPIGEKIFKEIVKPNPNVVMVLSGHYHSAMRKTDELDDD 1128
|||||||||||||||:.||.::||.:||:|.|.|||||.|.||.||||||
BPUM_3141 1084 FHEYLLVSGNRSPIGDLIFHQVVKRHPNIVAVFSGHYHGASRKVDELDDD 1133
BSNT_05334___ 1129 GDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDMVHVSTYSPYVKD 1178
.|||.||.|:|:|||||.|||||||::||:..||..:.:|.:|||||:..
BPUM_3141 1134 KDGKVDRKVYQILADYQSGPEGGQGFMRLITADQEKNQLHFTTYSPYMNQ 1183
BSNT_05334___ 1179 KNYYDTDTYGNKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKREQVK 1228
:::||..|||::||||:.:||.|::|.|:|:|||.|||||..:|:...:|
BPUM_3141 1184 EHFYDPKTYGDQDEFSIDVDLTPKMKSVKTNYFELNVYTNVLIGEARGIK 1233
BSNT_05334___ 1229 SGDTAEFRWDHLEPQSVYYWYIIVEDSFNGKTKSPIWKFKTKKE---TYR 1275
||..|.|.|..|:|...||||.:.:|.|.||..|||||..||.: |:.
BPUM_3141 1234 SGKKASFEWKGLKPNEDYYWYTVAKDRFQGKAISPIWKIHTKDDREATFL 1283
BSNT_05334___ 1276 PAPDQFDFRHVSNP 1289
..
BPUM_3141 1284 RK------------ 1285
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